FastQCFastQC Report
Fri 9 Jun 2017
SRR1178000_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178000_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19200172
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC16000.016.0451371
ACTAGCC69250.015.841065
CGTATGC74350.015.51901644-45
CCACTAG75800.014.5974333
ATCGGAA151400.014.3337492
GATCGGA149400.014.255981
CTAGCCT92650.013.7517821
TATGCCG88200.013.2168746-47
TATCTCG79650.013.18096838-39
CGGAAGA167000.013.1660144
GATCGTA26000.013.1507715
CGACAAT56550.013.0307291
TAGCTTA87250.012.85238134-35
TCGGAAG181450.012.091353
TAGCCTC105950.011.518652
CCCCACT135500.011.4730291
TGCCGTC103550.011.30509448-49
CGCCGAT12300.011.2088881
CGCGTAG6553.6190613E-810.8872861
ATCTCGT108000.010.64143240-41
Produced by FastQC (version 0.11.2)