FastQCFastQC Report
Fri 9 Jun 2017
SRR1178000_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178000_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19200172
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC68100.016.9513455
CCACTAG78750.015.0255653
GTCGAAC16550.014.3547731
CTAGCCT92300.014.2079591
CACTAGC93900.013.255794
CCCCACT136650.012.4827161
TAGCCTC103050.012.0833512
CGACAAT54800.011.0115241
CTCGTAT55700.010.9189051
CGCGTTT8308.913048E-1110.8767671
CCGGCAT40150.010.6507761
GCATACG18250.010.40798714-15
CGGGAAT27850.010.4070821
TCGTATG48600.010.3658222
GATCGTA25450.010.079785
CGGAAAC72850.09.978981
CTTAGAT57750.09.9553771
CGCGATT5254.0972456E-59.9553761
CGAAAAT39850.09.6578621
CGCAGAT36700.09.5805391
Produced by FastQC (version 0.11.2)