FastQCFastQC Report
Fri 9 Jun 2017
SRR1178002_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178002_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18844950
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC66800.018.271235
CCACTAG80500.016.1055953
CGACAAT52450.014.68481351
CACTAGC91500.014.11754
CTAGCCT87600.014.0027891
CCCCACT136950.012.3243851
GATCGTA26150.011.8046425
TAGCCTC107250.011.5564797
CGGGTAT25150.011.1535451
CGGGCAT44400.011.0294331
GTCGAAC15750.010.5654121
CTTAGAT61550.010.5053321
CGCGAAT16200.09.9784431
CGGCGAT17700.09.938651
CGGAAAT24200.09.8232131
CGGAAAC81500.09.7422161
CGCGAAC7351.9520303E-79.7029291
CGTATGA57850.09.6869853
CGGGAAT29450.09.6864561
CCGGCAT39750.09.5686741