FastQCFastQC Report
Fri 9 Jun 2017
SRR1178003_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178003_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24721331
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC98650.015.85981744-45
ACTAGCC92250.015.8031125
CCACTAG104400.014.600753
TATGCCG116250.013.72458946-47
GATCGTA32800.013.4641735
CTAGCCT117400.013.1209221
GATCGGA184950.012.6216051
ATCGGAA188550.012.5170062
CACTAGC123850.012.3844614
CGATGTA131700.012.03188734-35
TCACCGA129250.011.86160730-31
CCCCACT176900.011.6102971
TGCCGTC134900.011.53180648-49
GTCGAAC21450.011.5255991
CACACGT140300.011.50699612-13
CGGAAGA212200.011.5023974
CACGTCT147950.011.08862814-15
ATCTCGT141650.011.04679340-41
TATCTCG105200.010.90460438-39
CGCGAGT10550.010.8154911
Produced by FastQC (version 0.11.2)