FastQCFastQC Report
Fri 9 Jun 2017
SRR1178003_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178003_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24721331
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC89850.016.1725355
GATCGTA34750.015.8517765
GTCGAAC19000.014.7672081
CCACTAG104300.014.71538453
CTAGCCT113350.013.8869371
CGCCGTA41100.013.11164546-47
CACTAGC122700.012.3864034
CGCGTAT5503.7622158E-812.1050141
CGACGAT19750.012.0393461
GGATCGT48400.011.9716574
CCCCACT182850.011.8595951
CGCCGAT16850.011.5713481
CCGCAAT25950.011.178731
CCGGCAT52650.011.0194891
CGCGTTT10150.010.7761051
TAGCCTC144700.010.5495912
CCGGGAT80300.010.1269931
GGGATCG68900.010.06901553
CGATTAG27600.09.8085674
CTCGTAT76750.09.7279481
Produced by FastQC (version 0.11.2)