FastQCFastQC Report
Fri 9 Jun 2017
SRR1178005_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178005_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755734
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC86350.015.03971944-45
GATCGTA30900.013.8310825
TATGCCG95450.013.6808646-47
CTAGCCT110050.013.6511571
CGACAAT69850.013.3401931
GATCGGA173000.013.3280961
ATCGGAA176150.013.0746862
ACTAGCC85600.013.0366565
CCACTAG95700.012.603583
TAGCTTA103700.012.48589134-35
CGCGTAT6904.0017767E-1112.4021191
CGATTAG27400.012.1316434
TATCTCG95250.012.018970538-39
CGGAAGA199500.011.8062214
TAGCCTC127850.011.2913272
TCGGAAG210100.011.2557753
CGGGAAT38050.010.8701891
TGCCGTC124550.010.60222748-49
CCCCACT174900.010.5466471
GGATCGT45450.010.3436414
Produced by FastQC (version 0.11.2)