FastQCFastQC Report
Fri 9 Jun 2017
SRR1178005_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178005_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755734
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC83350.013.5592945
CTAGCCT109550.012.849971
CGATTAG29550.012.696684
CGCCGAT17150.012.4787531
GATCGTA30100.012.4631065
CCACTAG97900.012.4255793
CGACAAT63450.012.1424461
CTCGTAT66700.011.122991
CGGGAAT38150.010.9701081
GTCGAAC21000.010.870381
TAGCCTC123800.010.64096452
CACTAGC116650.010.5447334
CTCGGAT77850.010.201891
CGGAAAT37600.09.9922211
CCGGAAT39650.09.8354321
GGATCGT43150.09.7955714
CCCCACT180200.09.7913341
CTGAAAT228700.09.6280341
CTCCGAT81550.09.5057511
CTCGATT41750.09.3407151
Produced by FastQC (version 0.11.2)