FastQCFastQC Report
Fri 9 Jun 2017
SRR1178007_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178007_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16955044
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT59300.013.794941
GTCGAAC13550.012.9869911
ACTAGCC57000.012.9127825
CCACTAG64800.012.0941933
CTAGCCT72250.011.0590421
CGCGTAT5656.932005E-710.9431161
CGGAAAT25750.010.5279471
CGGGGTT32450.010.4061421
CTCGATT29350.09.8847981
CGACGAT17000.09.7918611
CCCCACT114350.09.7325311
CGGGCAT37300.09.6906041
CTTAGAT51500.09.5120931
CGCGAGT8652.1746018E-89.3471441
CGAATAT56700.09.3107791
CGCCGAT12800.09.2891531
GATCGTA23550.09.275345
CACTAGC82750.09.1241434
CTGGAAT174000.08.9654221
CGCGATT5951.5905549E-48.792691