FastQCFastQC Report
Fri 9 Jun 2017
SRR1178007_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178007_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16955044
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT58700.016.6077211
CCACTAG63200.014.1424533
ACTAGCC62600.014.109615
GATCGTA20750.012.1292565
CGCGTAT3952.4938627E-512.03918651
CGGAAAT25250.011.8651551
CGCGTGT10100.011.7709881
CTAGCCT74650.011.7043296
GTCGAAC15600.011.5838591
CCCCACT123400.011.329911
CACTAGC84300.010.8721744
CGCCGAT13000.010.60837651
CGCGAGT7804.0072337E-910.3645061
TAGCCTC91250.09.8872187
CGGGAAT23350.09.7757171
CGGGTAT20050.09.7244211
CTCGTAT40700.09.5810631
CGGGCTT42500.09.287171
CTTAAGT74250.09.2867941
CGGAAAC54250.09.2041531
Produced by FastQC (version 0.11.2)