FastQCFastQC Report
Fri 9 Jun 2017
SRR1178030_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178030_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19940249
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT92950.014.2599831
GTCGAAC18150.012.94829751
CGCGTAT5654.811227E-912.4784921
GATCGTA23600.012.3840215
ACTAGCC67950.011.9322135
TAGCCTC106500.011.817992
CGCCGAT12550.011.6101351
CGGGAAT30500.011.4038421
CCGGGAT64050.011.3744891
CGTATGC35600.011.22829644-45
GATCGGA534100.011.21819594
CGACAAT52500.010.8329161
CCACTAG74400.010.6443293
CGATTAG23900.010.2562224
ATCGGAA487150.010.19641994
AATCGTG24400.010.0460535
CGAATAT52100.09.8334991
CCGGCAT41150.09.8230861
CGGGTAT26000.09.7620211
AGATCGG645900.09.73478294
Produced by FastQC (version 0.11.2)