FastQCFastQC Report
Fri 9 Jun 2017
SRR1178030_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178030_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19940249
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC70800.015.081125
GATCGTA24850.014.9534595
CCACTAG76150.014.82013
CGCGAAT17600.013.3586551
GTCGAAC16100.012.5587961
CACTAGC93950.011.8156114
AGCGTCG145300.011.64248294
CGTCGTG107550.011.54620994
CTAGCCT94400.011.4104936
CGCGTAT6252.1322194E-811.2853921
GATCGGA497000.011.11632994
GGATCGT37450.010.9271874
GCGTCGT113100.010.85532194
TAGCCTC100350.010.6870647
CCGGGAT64400.010.0032271
ATCGGAA456950.09.93708794
CCCCACT158700.09.8074581
TAGATCG89950.09.79401394
CCGGCAT42850.09.7666251
AAGAGCG257200.09.76557594
Produced by FastQC (version 0.11.2)