FastQCFastQC Report
Fri 9 Jun 2017
SRR1178034_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178034_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16506176
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC14250.014.1797671
GATCGTA21150.013.7694485
CTAGCCT73050.013.1227671
CGCGAAT15750.012.5309551
CGCGTAT5504.4416083E-811.9613681
CGACAAT39650.011.4959271
CGTATGC30550.011.400454544-45
ACTAGCC49850.010.8359735
CGGGTAT20450.010.5701211
GATCGGA355450.010.55359594
GGATCGT30800.010.5228564
TAGCCTC81800.010.5083122
CCGGAAT26600.010.2461761
CCGCGAT11650.010.0839231
AGATCGG428300.09.99876594
ATCGGAA329850.09.91902294
GAGATCG241100.09.90472894
TATCTCG36100.09.8514838-39
CCGCGTT11550.09.764381
CGGGAAT29650.09.6676441
Produced by FastQC (version 0.11.2)