FastQCFastQC Report
Fri 9 Jun 2017
SRR1178034_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178034_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16506176
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA14600.019.2139765
GTCGAAC11850.016.1193221
ACTAGCC41800.016.0626375
CTCGTAT30900.015.2280451
CTAGCCT58650.013.9806031
ATCGTAC25000.013.4283656
GGATCGT22150.013.079984
CACGAAT48250.012.9386441
CCACTAG54700.012.6914563
CACTAGC57700.012.672484
CGACAAT29650.012.4131981
TCGTATG28800.012.2944872
TAGCCTC65500.012.2342242
TGTGCGA42750.012.1557748
CGTACTA19800.012.0775398
CGAATTG49150.011.97314553
GTGCGAA44000.011.6014049
GAATCGA25000.011.4049124
CTGGAAT118600.011.2740131
GCGGAAT31650.010.8927951
Produced by FastQC (version 0.11.2)