FastQCFastQC Report
Fri 9 Jun 2017
SRR1178040_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178040_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16528376
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA20900.015.2830655
CGCGTAT4755.7170837E-913.8501051
CGTATGC33100.013.36526244-45
CGCCGAT11550.013.019271
CGGGAAT25600.012.665661
CTAGCCT73900.012.208871
TCACGCC52550.011.48849130-31
TATGCCG36700.011.46974246-47
GGATCGT30000.011.4300844
GTCACGC48750.011.27446328-29
CGATTAG18700.011.0524594
GATCGGA400950.010.97432594
TATCTCG42900.010.92463338-39
CGCAAAT18100.010.9040891
ATCGTAC31750.010.6521346
AGATCGG482850.010.41750494
AGTCACG59750.010.33885628-29
GGGATCG48200.010.3301823
CCGGAAT26200.010.2233271
TAGATCG73700.010.14195894
Produced by FastQC (version 0.11.2)