FastQCFastQC Report
Fri 9 Jun 2017
SRR1178040_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178040_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16528376
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA20400.013.8418475
ACTAGCC57950.012.7502635
AGCGTCG119000.011.88147294
CCACTAG66200.011.4443333
TAGATCG73500.011.30074994
GATCGGA383250.011.09202394
ATCGTAC27850.010.6460466
CGTCGTG87200.010.48851994
GGATCGT31000.010.32333854
GTCGAAC12800.010.2930511
ATCGGAA351700.09.99072994
CTCGTAT48750.09.9416551
CTAGCCT75300.09.8124546
CACTAGC80900.09.7731314
CGCCGAT10609.094947E-129.7659151
AGATCGG471050.09.70808794
CGGGTAT21450.09.6520921
ATCGTGC22450.09.643056
GAGATCG245700.09.62891594
CGCCAAT29750.09.4898721
Produced by FastQC (version 0.11.2)