FastQCFastQC Report
Fri 9 Jun 2017
SRR1178044_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178044_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17626121
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC64200.020.88909344-45
TATGCCG76350.017.46250246-47
TATCGTA26250.015.78962540-41
ATCGTAT30000.015.37452242-43
GACCAAT111150.015.11040134-35
ATATCGT33050.014.74984240-41
AATCTCG72850.014.29429738-39
TGCCGTC90400.013.82368648-49
AATATCG36000.013.54683838-39
ATCTCGT79950.013.43181840-41
TCGTATG97250.013.11524742-43
GATCGTA20600.012.99766355
GTCGAAC17050.012.1256961
CTAGCCT69900.012.0996761
GTATGCC107100.011.64303544-45
CTCGTAT85700.011.45043642-43
CGGGAAT28550.011.3558951
TCTCGTA81250.011.30393740-41
CGACAAT43350.011.2725611
AGTCACT180750.010.88924328-29
Produced by FastQC (version 0.11.2)