FastQCFastQC Report
Fri 9 Jun 2017
SRR1178056_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178056_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15213212
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29400.015.20783444-45
CGACAAT46750.015.1749371
GATCGTA20200.014.1836275
GTCGAAC13150.012.147441
TATGCCG36300.012.04851746-47
CGGGAAT22200.011.8513171
GTCGAAT15300.011.3616621
ATCGTAC26800.011.2164126
CGCGTTT7151.1641532E-911.17054751
TAGATCG85150.010.98752794
CGGGTAT17600.010.677731
CTAGCCT59950.010.6581371
GACCAAT64300.010.62417834-35
ATCGGAA474650.010.42014594
CGATTAG17250.010.3467344
GATCGGA519100.010.34300194
AGATCGG601050.010.11391794
TATCGTA14950.010.08025840-41
CCGGCAT30950.010.018781
GAGATCG323650.09.96506294
Produced by FastQC (version 0.11.2)