FastQCFastQC Report
Fri 9 Jun 2017
SRR1178056_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178056_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15213212
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA19700.013.7400975
GGATCGT26450.012.0610824
CGCCGAT11250.012.0346171
CGTCGTG109450.011.86941394
CGACAAT49700.011.7036851
AGCGTCG151350.011.58767794
GTCGAAC12100.011.189211
CTAGCCT60650.010.9135031
GCGTCGT122700.010.77673794
ATCGTAC26700.010.6624326
TAGATCG96800.010.59263394
ATCGGAA454950.010.53473694
CTCGAAC32100.010.4662421
GATCGGA499050.010.37545594
CGGGAAT25300.010.3063261
GAGCGTC186700.010.28828694
TCGGAAG429100.010.05567994
GCGGAAT39850.09.9407651
CGAATAT39500.09.90191
CTCGTAT39550.09.8893821
Produced by FastQC (version 0.11.2)