FastQCFastQC Report
Mon 5 Jun 2017
SRR1178060_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178060_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15903449
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC15150.016.4365391
GATCGTA19750.013.0796645
CGTATGC23800.012.06317244-45
CGGGTAT16650.011.8517431
GCGGAAT31700.011.5606571
GATCGGA420000.011.00386294
AGATCGG512800.010.63532894
GGATCGT29450.010.525914
CGACAAT26050.010.4608611
CGGGAAT25800.010.3801181
CGGAAAT25550.010.2977961
CGCGTTA6051.9531617E-610.0956691
ATCGGAA381000.09.93371894
CCGCAAT14700.09.9081261
TAGATCG76000.09.89799794
CGAATTG83800.09.8646253
CCGGCAT31100.09.8197431
TCGGAAG374800.09.696630594
ACACGTC100650.09.62264394
GAGATCG275650.09.51737494
Produced by FastQC (version 0.11.2)