FastQCFastQC Report
Fri 9 Jun 2017
SRR1178065_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178065_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31741371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC108400.016.2728585
CGTATGC117900.016.04683744-45
CGACAAT93150.014.7736881
CTAGCCT145300.014.6640711
CCACTAG124000.014.4559813
TAGCTTA145100.013.68940934-35
TATGCCG139600.013.63811746-47
TATCTCG134450.013.56843838-39
ATCGGAA266600.013.5185762
GATCGGA270400.013.3025941
CGGAAGA287300.012.8581744
GTCGAAC26350.012.0661311
GATCGTA44500.011.8280495
TAGCCTC172050.011.8244262
TGCCGTC165800.011.76939448-49
TCGGAAG311900.011.6767863
CGGGAAT48300.011.3968461
ATCTCGT176100.011.0704840-41
CCCCACT219550.010.958431
CACGTCT189750.010.82080714-15
Produced by FastQC (version 0.11.2)