FastQCFastQC Report
Fri 9 Jun 2017
SRR1178065_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178065_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31741371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC115800.016.2359265
CCACTAG126800.015.9851843
GTCGAAC25250.015.8104891
CTAGCCT150800.014.7178011
CGACAAT90800.013.6609871
GATCGTA41150.012.8068895
CGCGTAT7105.456968E-1212.7181121
CACTAGC157550.012.6545924
CCCCACT221200.012.3755581
TAGCCTC174450.012.3138782
GCATACG29100.011.66037714-15
CCGGCAT67350.011.6432371
CGCCGAT20000.011.4061371
CGCGTTT11150.010.6559591
CGGAAAC117900.010.440311
CGCGGTT29800.010.3663181
CGGGAAT47500.010.0053841
CGGAAAT41950.09.9696091
GGATCGT60150.09.8648764
CGCGTGT17900.09.8237231
Produced by FastQC (version 0.11.2)