FastQCFastQC Report
Fri 9 Jun 2017
SRR1178066_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178066_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56193870
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG235750.056.223791
ATCGGGA245350.051.3130042
GAGCGTC886200.038.14569
AGAGCGT904450.038.0798158
AAGAGCG1005600.037.788617
GATCGGA1152800.036.920981
ATCGGAA1148750.036.819552
AGACGGC428150.035.532579
CGGAAGA1159850.034.0395934
GCATACG187300.033.6643114-15
TCGGGAG245050.033.626113
TCGGAAG1192150.032.7600863
GAGAGCG264700.031.9320457
GAGCGGC280800.031.3530359
GAAAGCG251750.031.2155367
CGGGAGA287050.031.1102094
CGCCGTA520050.030.85237946-47
AGAGCGG304100.030.3256668
CGGCGTG225950.029.82719812-13
TCGGGGG622950.029.52325638-39
Produced by FastQC (version 0.11.2)