FastQCFastQC Report
Fri 9 Jun 2017
SRR1178067_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178067_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41130937
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT192900.015.3283881
GATCGTA52750.014.7389855
ACTAGCC138100.013.69700055
CCACTAG156750.013.1561223
GTCGAAC33350.013.0928031
CGCGTTT16450.012.9833661
TAGCCTC223000.012.9042472
CGCGTAT9100.011.9957421
CGACAAT99950.011.966261
CGCGATT10600.011.64147951
CGGGAAT57200.010.8696581
CACTAGC194200.010.5702414
GGATCGT76450.010.541874
CCCCACT279600.010.3715741
CGCGGTT40050.010.309971
CGGGCAT96500.010.2300251
CGATTAG50850.010.162064
CTGAAAT425500.010.139231
GGGATCG101050.09.9928533
CTCCGTT76850.09.6960861