FastQCFastQC Report
Fri 9 Jun 2017
SRR1178067_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178067_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41130937
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC148850.014.9364465
CCACTAG161100.014.9082223
CTAGCCT194050.014.6152131
GATCGTA51600.013.1654785
CCCCACT289400.012.3606381
GTCGAAC33400.012.0897391
CGACAAT97850.012.0887951
TAGCCTC221900.011.9673772
CGATTAG46850.011.9571834
CACTAGC203100.011.5003654
CGACGAT36750.010.599881
CTGAAAT412800.010.1731871
GGATCGT78450.09.9244714
CGGGCAT97450.09.6522331
CGCCGAT27000.09.5011121
CTCGTAT112350.09.4715141
CGCAAAT44350.09.4261311
GCTTTAA286800.09.3919155
GGGATCG104700.09.3841423
CTCGGGT117250.09.3593041
Produced by FastQC (version 0.11.2)