FastQCFastQC Report
Fri 9 Jun 2017
SRR1178068_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178068_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49061482
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC173800.014.74712144-45
TATGCCG186950.013.7994246-47
GTCGAAC40650.013.6551881
TAGCTTA209050.012.87392534-35
CTAGCCT218700.012.75561
GATCGGA370450.012.6659871
ATCGGAA372050.012.642632
TATCTCG189800.012.21200838-39
GATCGTA66650.012.0931285
CGACAAT135200.012.036211
CGCGTAT12750.011.9072691
CGGAAGA424450.011.551044
ACTAGCC164650.011.3167235
CGATTAG58800.010.886384
TGCCGTC248900.010.69917448-49
TCGGAAG455300.010.5184273
CCACTAG192750.010.4549423
TAGCCTC255450.010.4131952
CGCGTTT21450.010.395441
CACACGT277950.010.19217412-13
Produced by FastQC (version 0.11.2)