FastQCFastQC Report
Fri 9 Jun 2017
SRR1178068_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178068_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49061482
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT12550.014.0027221
ACTAGCC163650.013.331935
GATCGTA60350.013.3108495
CTAGCCT228950.013.235321
GTCGAAC39850.013.1104871
CGACAAT133000.012.2846051
CCACTAG201900.011.4633863
TAGCCTC258050.011.0801862
CGCCGTA79200.010.32383146-47
CGATTAG60500.010.2803344
CTCGTAT141950.010.1382191
CCCCACT359250.010.0742471
GGATCGT87850.010.0522324
CACTAGC232700.09.9974914
CGCGTTT23950.09.7172861
GGGATCG124500.09.7140473
CGGGCAT116650.09.6905111
CGGGAAT71550.09.6252661
CCGGCAT107100.09.5346211
CTGAAAT486350.09.2969921
Produced by FastQC (version 0.11.2)