FastQCFastQC Report
Fri 9 Jun 2017
SRR1178069_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178069_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27722265
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC21850.015.4436051
CGACAAT93800.015.0485761
CCACTAG101750.012.0831093
CTAGCCT114900.012.0782671
CGCGTAT5655.514812E-811.776641
ACTAGCC97500.011.6870625
CGCGTTT11800.011.277631
CCCCACT203500.011.0001421
CGGGAAT41500.010.9942331
AATCGTG34650.010.550825
CGACGAT27300.010.4455281
GATCGTA36100.09.8639945
CGATTAG34000.09.77331354
CGGGTAT34000.09.6452161
CGGGCAT64700.09.47604751
CGCGTAA4100.0055131729.2735911
TAGCCTC144150.09.1988042
CACTAGC131600.09.1982884
CGGAAAT42700.09.1269941
ATCGTGC34500.09.0815046
Produced by FastQC (version 0.11.2)