FastQCFastQC Report
Fri 9 Jun 2017
SRR1178071_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178071_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39403363
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT170850.012.8313431
ACTAGCC123350.012.57088855
CGACAAT103650.012.13170051
GATCGTA53750.012.0864695
CGTATGC138350.011.77130144-45
GTCGAAC37650.011.5949261
CCACTAG150650.011.4265673
CGGGAAT64000.010.9730371
CGCGTAT10900.010.883251
CGCGTTT15400.010.7843121
TAGCCTC198200.010.527552
ATCACGA162500.010.45684834-35
CTCGGAT139400.09.9395151
TATGCCG166600.09.81833246-47
GGATCGT77600.09.7165854
CGATTAG37700.09.6856264
CCGGCAT82000.09.6059281
CGGAAAT55750.09.4476381
CTGAAAT380150.09.3241821
CGTATGA110150.09.1270343
Produced by FastQC (version 0.11.2)