FastQCFastQC Report
Fri 9 Jun 2017
SRR1178071_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178071_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39403363
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC37850.015.0689071
ACTAGCC129750.013.7591885
GATCGTA47900.013.2825555
CCACTAG156750.012.3258863
CTAGCCT175100.012.1064021
CGACAAT105550.011.6179061
GGATCGT73150.010.57827954
CGGGAAT64400.010.4801831
CGCGTAT10550.010.3619581
CGCCGTA83300.010.31301146-47
CACTAGC189900.09.8744724
TAGCCTC203150.09.8031032
CGCGAGT18550.09.7365721
CTCGTAT97750.09.6275281
CCGGCAT84800.09.5283891
CCCCACT291800.09.5124851
CTCGGAT132050.09.4303821
CGTACTA63100.09.4053588
CTGAAAT383150.09.2913621
CGGCGAT32850.09.1153131
Produced by FastQC (version 0.11.2)