FastQCFastQC Report
Fri 9 Jun 2017
SRR1178072_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178072_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50021046
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT134450.013.1977851
CTAGCCT211950.012.5132071
CGTATGC191450.012.06118444-45
GTCGAAC51400.011.7227871
GATCGTA59250.011.6032095
ACTAGCC152550.011.0956945
ATCACGA222300.010.77219634-35
TATGCCG224100.010.51749646-47
CGCGTAT13400.010.2679431
TAGCCTC243850.010.1114072
CGGGCAT129400.010.0463091
CCACTAG189250.09.8215773
CGGGAAT80150.09.7080181
CTGAAAT494850.09.1467131
CGCCGAT32750.09.1268211
CGCGATT15150.09.0818771
CTCGGAT157950.09.041431
CTGGAAT537900.08.8645111
CGTATGA134350.08.8586413
CACGATC239800.08.8584836-37