FastQCFastQC Report
Fri 9 Jun 2017
SRR1178072_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178072_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50021046
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC51150.014.9503311
CGACAAT130250.014.5500921
ACTAGCC152400.013.6922435
CGCCGTA113300.012.54506446-47
CCACTAG192200.011.918863
CTAGCCT216400.011.6109831
GATCGTA61200.011.0288935
CGCGTAT14400.010.5549951
CACTAGC216550.010.4803024
CGGCGAT41350.010.4528921
CGTATCA114700.010.30499750-51
CTCGTAT128300.010.0696141
CCGTATC154300.09.79699248-49
CGCGATA12200.09.7330892
TAGCCTC251100.09.552462
GCCGTAT127700.09.52973148-49
CCCCACT348050.09.4708381
CTGAAAT496550.09.412451
CCGGCAT107850.09.2484661
GGATCGT88400.09.0232114
Produced by FastQC (version 0.11.2)