FastQCFastQC Report
Fri 9 Jun 2017
SRR1178073_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178073_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52175624
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC59500.014.2116841
CGCCGTA95850.013.67321846-47
GATCGTA66900.012.2130125
CGACAAT92550.011.4464531
ACTAGCC153250.010.9419485
CGCCGAT31850.010.8881961
CGTATCA95700.010.76125150-51
GGATCGT97300.010.2462384
CCGTATC138350.010.09071448-49
CTCGTAT132600.09.9238171
CCGGCAT109350.09.86164951
CGGGAAT82100.09.8366731
CCACTAG186600.09.7930873
CTGAAAT531700.09.6761881
GCCGTAT105300.09.58262248-49
CGATTAG52500.09.404424
CTCGATT93550.09.3436491
CTAGCCT206300.09.3260621
CGGCGAT35700.09.1817061
GCGGAAT102850.08.914471
Produced by FastQC (version 0.11.2)