FastQCFastQC Report
Fri 8 Jul 2016
G101M6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M6.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20491919
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC408540.19936639413809903TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG57450.033.04335443
GATCGGA60100.031.9236411
ATCACGA59000.031.81268334
CGTATGC60500.031.15937844
ACACGTC63000.030.9009213
ATCGGAA63550.030.2251952
CTCGTAT63850.029.66271642
ACGTCTG66500.029.4065115
CGTCTGA66100.029.28495816
ACGATCT66250.028.63007737
TCTCGTA69600.027.40450941
TCACGAT70250.026.96857535
CACGTCT72050.026.92768514
CACACGT72650.026.88722812
CACGATC71500.026.71251736
CATCACG75450.024.90551633
ATCTCGT76400.024.8236340
GATCTCG78650.023.94698139
CGGAAGA82500.023.9195944
GCACACG86800.022.68151311
Produced by FastQC (version 0.11.2)