FastQCFastQC Report
Mon 22 Jun 2015
G101_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101_M1.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8812824
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC351610.39897540220932587TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42750.038.2410621
TATCTCG42750.037.26125739
CGTATGC43150.037.0620844
TCGTATG43450.036.9580743
TCACCGA43850.036.57824330
CTCGTAT44050.036.10530542
ATCGGAA45350.035.8545572
ACACGTC45450.035.4769513
CGTCTGA45400.035.37066316
ACGTCTG45800.035.0137215
CACCGAT46100.034.98427631
CGATGTA46050.034.7843534
ACCGATG46800.034.32113332
TCTCGTA47500.033.71715541
CACGTCT48050.033.51149714
CCGATGT48250.033.2443133
CACACGT50050.032.4360912
GTCACCG49850.032.21978429
CGGAAGA51950.031.7616964
ATCTCGT51550.031.07100540
Produced by FastQC (version 0.11.2)