FastQCFastQC Report
Mon 22 Jun 2015
G101_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101_M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14000325
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC692480.4946170892461425TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC80450.038.99049444
TCGTATG80550.038.91477643
CTCGTAT82100.037.96628642
AATCTCG86050.036.43646639
TCTCGTA88850.035.15962641
ATCTCGT91200.034.2575640
GTAATCT100050.031.53763637
TAATCTC102600.030.64646738
TGTAATC109050.028.89445136
GATCGGA113050.027.9656121
CTTGTAA117100.027.0021634
ACACGTC117500.026.84613
TTGTAAT118600.026.75331735
ATCGGAA118200.026.7286242
CGTCTGA119100.026.42975216
AGTCACC121050.026.42817728
ACCTTGT120850.026.27286532
CCTTGTA121500.026.22276733
ACGTCTG121250.026.0518115
CACGTCT125650.025.10451714
Produced by FastQC (version 0.11.2)