FastQCFastQC Report
Fri 2 Jun 2017
G102C_M2_40bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG102C_M2_40bp.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences28451031
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10300.07.4302541
ATCGGAA11650.06.7129782
CGTCTGA15350.05.20487416
CGATGTA7303.751662E-65.125521734
ACACGTC15300.05.110841813
ACGTCTG16100.05.06799415
Produced by FastQC (version 0.11.2)