FastQCFastQC Report
Fri 2 Jun 2017
G102C_M5_40bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG102C_M5_40bp.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences29387053
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA12500.09.6571482
GATCGGA13200.09.5341291
CGTCTGA17850.07.523321616
ACACGTC18450.06.818020313
ACGTCTG20400.06.24959415
TCGGAAG24800.05.34740973
Produced by FastQC (version 0.11.2)