FastQCFastQC Report
Fri 2 Jun 2017
G111C_M5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111C_M5.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19973948
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC3800.021.49236333
ACGTCCG4950.016.15521232
GATCGGA6550.012.7145661
CACGACG2055.0622475E-912.43594415
GTCCGCA7050.011.10184834
ATCGGAA8250.010.5065672
ACGACGC2509.452924E-810.19742216
CGACGCT2601.763774E-69.15134917
CGTCTGA10500.08.74064716
ACACGTC11050.07.536537613
ACGTCTG12700.06.958905715
TCGGAAG12800.06.7717943
AGTCACG13400.06.600436728
GTCACGT13200.06.18561429
CACGTCT15850.05.79036414
CGGAAGA16500.05.6652494
Produced by FastQC (version 0.11.2)