FastQCFastQC Report
Wed 11 Mar 2015
G104C_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104C_M1.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16555854
Sequences flagged as poor quality0
Sequence length40-45
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG11915327.197043414371739TruSeq Adapter, Index 7 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG1255050.082.4718839
GATCATC1268700.081.4644536
ATCATCT1288150.080.43330437
AGATCAT1290200.080.2140535
TCATCTC1300300.079.7461638
AATCTCG10950.038.6352639
TATCTCG8400.036.40157339
GATCGGA3467300.036.027121
ATCGGAA3472050.035.975472
TCGGAAG3479800.035.8787233
CGTCTGA3463900.035.8760316
ACACGTC3466500.035.8708313
CGGAAGA3480900.035.85444
ACGTCTG3465850.035.84906415
CACGTCT3468400.035.82407414
GCACACG3477650.035.8079111
CACACGT3472100.035.8020412
GAACTCC3488350.035.6100921
ACCAGAT3399550.035.5762232
GTCTGAA3495000.035.555917
Produced by FastQC (version 0.11.2)