FastQCFastQC Report
Wed 11 Mar 2015
G104C_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104C_M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17313728
Sequences flagged as poor quality0
Sequence length40-45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG7294124.212911280574582TruSeq Adapter, Index 10 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG780400.080.89854439
GCTTATC792350.079.2148736
TTATCTC819600.077.4471938
AGCTTAT814900.077.1646235
CTTATCT818050.077.0591937
GATCGGA2195500.035.5867081
ATCGGAA2197800.035.5162472
CGTCTGA2182300.035.46721616
TAGCTTA2097900.035.4041434
TCGGAAG2205900.035.385833
ACGTCTG2187350.035.3803715
ACACGTC2193200.035.35313413
CGGAAGA2208400.035.318734
CACGTCT2195450.035.29382314
ACTAGCT2112400.035.2185532
GTCACTA2118800.035.213829
CACACGT2203350.035.20259512
CACTAGC2114000.035.20131331
GCACACG2208900.035.1788611
TCACTAG2115200.035.16337630
Produced by FastQC (version 0.11.2)