FastQCFastQC Report
Wed 11 Mar 2015
G104_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M1.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7151887
Sequences flagged as poor quality0
Sequence length45
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG119153216.66038627288155TruSeq Adapter, Index 7 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1303950.038.8412781
ATCGGAA1306700.038.745722
ACACGTC1300450.038.6333213
CATCTCG1248350.038.6280339
CGTCTGA1300000.038.61888516
TCGGAAG1310550.038.615533
ACGTCTG1300350.038.593515
CGGAAGA1311750.038.5742574
CACGTCT1301550.038.56471614
GCACACG1307850.038.52802711
CACACGT1302800.038.51574312
GATCATC1254800.038.37289436
ACCAGAT1263750.038.276932
GAACTCC1317900.038.24566321
AGATCAT1261300.038.22619235
GTCTGAA1314050.038.20044717
AGTCACC1283500.038.17095628
CCAGATC1262350.038.16833
CAGATCA1261800.038.14145334
TCTGAAC1318550.038.1410518
Produced by FastQC (version 0.11.2)