FastQCFastQC Report
Wed 11 Mar 2015
G104_M1b.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M1b.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9403967
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA207559022.071430067757575TruSeq Adapter, Index 7 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2158700.033.8626821
ATCGGAA2160200.033.832092
CGTCTGA2158900.033.7831716
TCGGAAG2164050.033.762653
CGGAAGA2163050.033.7601854
ACACGTC2161500.033.74489213
ACGTCTG2161600.033.73782715
CACGTCT2162650.033.72301514
CACACGT2165800.033.69908512
GCACACG2167350.033.6883211
GAACTCC2166100.033.57278421
GTCTGAA2174450.033.53029317
TCTGAAC2176300.033.49231718
ACCAGAT2130850.033.4915532
CAGATCA2129400.033.48162534
AGAGCAC2183550.033.4790468
AACTCCA2171650.033.4595822
AGTCACC2135400.033.44964228
GAGCACA2185300.033.446799
CCAGATC2140650.033.4335233
Produced by FastQC (version 0.11.2)