FastQCFastQC Report
Wed 11 Mar 2015
G104_M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M2.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9874759
Sequences flagged as poor quality0
Sequence length45
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG101042910.23244212846106TruSeq Adapter, Index 8 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1135800.038.8454441
ATCGGAA1137750.038.7755622
AATCTCG1063100.038.72261439
CGTCTGA1134700.038.5844316
ACACGTC1135350.038.57646613
ACGTCTG1134900.038.55357715
TCGGAAG1145300.038.5216523
CACGTCT1136950.038.5050314
GCACACG1141350.038.47274411
CGGAAGA1147150.038.4510274
CACACGT1137900.038.44033412
TTGAATC1076250.038.07929636
GAACTCC1155650.037.97904621
GAATCTC1083850.037.9077238
GTCTGAA1156200.037.87854817
TGAATCT1083800.037.8230337
ACTTGAA1082200.037.8122234
CTTGAAT1084500.037.80941435
CACTTGA1084300.037.80276533
TCCAGTC1150550.037.77969725