Basic Statistics
Measure | Value |
---|---|
Filename | G104_M2.fastq |
File type | Conventional base calls |
Encoding | Illumina 1.5 |
Total Sequences | 9874759 |
Sequences flagged as poor quality | 0 |
Sequence length | 45 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG | 1010429 | 10.23244212846106 | TruSeq Adapter, Index 8 (100% over 45bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 113580 | 0.0 | 38.845444 | 1 |
ATCGGAA | 113775 | 0.0 | 38.775562 | 2 |
AATCTCG | 106310 | 0.0 | 38.722614 | 39 |
CGTCTGA | 113470 | 0.0 | 38.58443 | 16 |
ACACGTC | 113535 | 0.0 | 38.576466 | 13 |
ACGTCTG | 113490 | 0.0 | 38.553577 | 15 |
TCGGAAG | 114530 | 0.0 | 38.521652 | 3 |
CACGTCT | 113695 | 0.0 | 38.50503 | 14 |
GCACACG | 114135 | 0.0 | 38.472744 | 11 |
CGGAAGA | 114715 | 0.0 | 38.451027 | 4 |
CACACGT | 113790 | 0.0 | 38.440334 | 12 |
TTGAATC | 107625 | 0.0 | 38.079296 | 36 |
GAACTCC | 115565 | 0.0 | 37.979046 | 21 |
GAATCTC | 108385 | 0.0 | 37.90772 | 38 |
GTCTGAA | 115620 | 0.0 | 37.878548 | 17 |
TGAATCT | 108380 | 0.0 | 37.82303 | 37 |
ACTTGAA | 108220 | 0.0 | 37.81222 | 34 |
CTTGAAT | 108450 | 0.0 | 37.809414 | 35 |
CACTTGA | 108430 | 0.0 | 37.802765 | 33 |
TCCAGTC | 115055 | 0.0 | 37.779697 | 25 |