FastQCFastQC Report
Wed 11 Mar 2015
G104_M2b.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M2b.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences12502734
Sequences flagged as poor quality0
Sequence length40
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA169526413.559146343511747TruSeq Adapter, Index 8 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1807050.033.901481
ATCGGAA1808650.033.8649142
ACACGTC1811600.033.7290713
CGTCTGA1810600.033.726116
TCGGAAG1816200.033.7147753
ACGTCTG1812100.033.69443515
CGGAAGA1818100.033.664724
CACGTCT1815200.033.64719414
CACACGT1817900.033.62246312
GCACACG1818350.033.62162411
GAACTCC1827050.033.3484421
AGAGCAC1835700.033.344738
GTCTGAA1832400.033.3222417
ACTTGAA1763600.033.32076334
GAGCACA1839750.033.2759489
CACTTGA1779200.033.22733733
TCTGAAC1837000.033.2234718
AACTCCA1834050.033.2016922
GTCACAC1772100.033.15709729
AGCACAC1845200.033.15463310
Produced by FastQC (version 0.11.2)