FastQCFastQC Report
Wed 11 Mar 2015
G104_M3b.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M3b.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences10866107
Sequences flagged as poor quality0
Sequence length40
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA201780318.569695660092435TruSeq Adapter, Index 9 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2114600.033.893181
ATCGGAA2117600.033.8628272
TCGGAAG2121200.033.802953
CGTCTGA2116650.033.795316
ACACGTC2118300.033.78422513
ACGTCTG2118000.033.77215615
CGGAAGA2122850.033.757464
CACGTCT2119900.033.73787714
CACACGT2123300.033.7214812
ACGATCA2070950.033.70134732
GCACACG2125200.033.70044311
CGATCAG2079650.033.693633
AGTCACG2072550.033.68763428
TCACGAT2071200.033.67429730
GTCACGA2071400.033.66776329
CACGATC2073050.033.63932831
GATCAGA2079950.033.55960534
GAACTCC2127550.033.53005221
AGAGCAC2142900.033.464928
GTCTGAA2140100.033.42568617
Produced by FastQC (version 0.11.2)