FastQCFastQC Report
Wed 11 Mar 2015
G104_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7598629
Sequences flagged as poor quality0
Sequence length45
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG7294129.599257971405105TruSeq Adapter, Index 10 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG777250.038.40880239
GATCGGA830250.038.2564741
ATCGGAA831850.038.1506422
TAGCTTA779900.038.01054434
GCTTATC781300.037.98739236
TCGGAAG837250.037.9208873
CGTCTGA828400.037.9009416
ACGTCTG828600.037.8800315
GTCACTA791200.037.866129
ACTAGCT785750.037.8607732
ACACGTC830800.037.84148413
CACTAGC787100.037.8404231
CGGAAGA838750.037.8367964
AGCTTAT786100.037.82494435
TCACTAG788350.037.74827230
CACGTCT832550.037.74320614
GCACACG839150.037.62293211
CACACGT835700.037.61727512
CTAGCTT790600.037.59645533
TTATCTC798400.037.59418538