FastQCFastQC Report
Wed 11 Mar 2015
G104_M4b.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M4b.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9715099
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA127424113.116088678046408TruSeq Adapter, Index 10 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1358550.033.50170516
GATCGGA1371400.033.4666981
ATCGGAA1370750.033.449082
ACGTCTG1363350.033.39747215
TAGCTTA1322300.033.36338834
CACGTCT1365800.033.35747514
ACACGTC1367600.033.3458913
TCGGAAG1375450.033.3248983
CGGAAGA1375100.033.2962954
CACACGT1371500.033.25850712
CACTAGC1329750.033.20459431
GCACACG1375800.033.19073511
ACTAGCT1331000.033.17979432
GTCACTA1332400.033.17427429
TCACTAG1332000.033.14084630
GAACTCC1369450.033.102521
TCTGAAC1376950.033.03512218
CTAGCTT1345650.033.00174333
GTCTGAA1379750.032.9914117
AACTCCA1375550.032.92727722
Produced by FastQC (version 0.11.2)