FastQCFastQC Report
Wed 19 Jul 2017
G110_M11.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M11.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595815
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC351720.5332472181224003TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA43650.041.2683941
CTCGTAT43200.039.41074442
ACGGCTA44750.039.25813332
ATCGGAA46300.039.049782
CGTCTGA47200.038.58215716
CGGCTAC46050.038.19767433
CGTATGC44350.037.9384144
ACGTCTG48450.037.49737515
AGTCACG48150.037.02118728
ATCTCGT46250.036.96061340
TCACGGC49900.035.3382630
CGGAAGA52650.035.218444
GCACACG53700.034.24127211
GCTACAT51950.033.9437835
CACGGCT52000.033.91139631
TCGTATG50200.033.73841543
GTCACGG52900.033.53362729
CATCTCG52200.033.38467439
TCGGAAG56200.032.3680653
TCTCGTA53350.032.1208341
Produced by FastQC (version 0.11.2)