FastQCFastQC Report
Wed 19 Jul 2017
G110_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17088339
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9550.011.0551571
CTCGTAT8700.010.61881742
ACGTTCG2751.3402587E-610.39658456
CGGGCTA9600.09.8519971
CGGGGTT22050.09.4763711
ATACGTC31650.09.46009131
GCCGGAT12800.09.4510431
GTCGGAT9850.09.1553441
TCATACG33800.09.05175629
TACGTCA32600.09.04945532
TCGGATC14350.09.0433572
CGGGTTT16300.09.0409531
CGTTCGA3452.4783385E-68.9245787
CATACGT34850.08.71753530
CGCCCTT19850.08.6429331
ATCGGAA12500.08.6221512
CGTATGC11250.08.60244844
GCGGAAT7950.08.5767281
TTACGAC5202.3737812E-98.4598245
GTCGGTT16500.08.39814951
Produced by FastQC (version 0.11.2)