FastQCFastQC Report
Wed 19 Jul 2017
G110_M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13395345
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC135700.10130384846377605TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18900.033.1702742
TCGTATG19500.032.3744143
CGTATGC19900.031.61195444
TATCTCG20550.031.15898539
GATCGGA20850.030.1701341
CGATGTA23950.027.20816434
CACCGAT23800.027.10295931
TCACCGA24300.026.54439430
ATCGGAA24500.025.6761682
ACCGATG25300.025.5830832
TCTCGTA24700.025.56103541
ATCTCGT26500.024.241640
ACACGTC27350.023.96466413
CGTCTGA27650.023.46557416
GTCACCG27650.023.32276729
CCGATGT28400.022.8680833
ACGTCTG30000.021.70099415
TCGGAAG34400.019.310783
CACACGT35200.019.05751812
CACGTCT35100.018.86130714
Produced by FastQC (version 0.11.2)